Metode Isolasi Squalen dari Minyak Hati Ikan Hiu

Penulis : Silverius Yohanes & Maximus M. Taek Editor: Maximus M. Taek

Metode Isolasi Squalen dari Minyak Hati Ikan Hiu

Authors

Penulis : Silverius Yohanes & Maximus M. Taek Editor: Maximus M. Taek

Synopsis

Salah satu senyawa penting yang terkandung dalam minyak ikan adalah squalen (squalene, skualena). Squalen memiliki banyak manfaat bagi manusia, dan diaplikasikan secara luas dalam berbagai keperluan. Beberapa manfaat squalen antara lain untuk kesehatan kulit, yakni membantu memperbaiki kerusakan kulit akibat sinar UV dan mengurangi tanda-tanda penuaan seperti garis halus dan kerutan. Squalen juga berfungsi menangkal radikal bebas, sebagai antioksidan yang membantu melindungi sel-sel tubuh dari kerusakan oksidatif yang menyebabkan penyakit degeneratif. Squalen juga memiliki sifat antiinflamasi yang dapat membantu mengurangi peradangan dalam tubuh. Manfaat lain dari squalen adalah membantu memelihara dan meningkatkan kesehatan jantung, dan memperkuat sistem kekebalan tubuh. Berbagai produk perawatan kulit seperti krim, lotion dan serum, dan berbagai formulasi obat-obatan, vaksin dan suplemen kesehatan banyak menggunakan squalen sebagai bahan aktifnya.

Buku ini menguraikan tentang salah satu cara sederhana untuk mendapatkan squalen dari minyak hati ikan hiu. Dalam buku ini diuraikan cara dan tahapan proses isolasi untuk mendapatkan squalen murni dari minyak hati ikan hiu, mulai dari ekstraksi minyak hati ikan hiu, hidrolisis minyak, pemisahan squalen dan pemurnian squalen, serta identifikasi dan karakterisasi squalen dari minyak hati ikan hiu.

ISBN: 978-623-8324-17-0

References

A. Andrieş, I. Popa, N. Elena Băbeanu, S. Niţă, and O. Popa, “Squalene- natural resources and applications,” Farmacia, vol. 62, no. 5, pp. 840–862, 2014.

A. Fernandez-Cuesta, L. Leon, L. Velasco, and R. De la Rosa, “Changes in squalene and sterols associated with olive maturation,” Food Research International, vol. 54, no. 2, pp. 1885–1889, 2013.

A. L. Ronco, E. De Stéfani, and A. Ronco, “Squalene: a multi-task link in the crossroads of cancer and aging,” Functional Foods in Health and Disease, vol. 3, no. 12, pp. 462–476, 2013.

A. Lanzón, A. Guinda, T. Albi, and C. De la Osa, “Método rápido para la determinación de escualeno en aceites vegetales,” Grasas y Aceites, vol. 46, no. 4-5, pp. 276–278, 1995.

A. Nakazawa, Y. Kokubun, H. Matsuura et al., “TLC screening of thraustochytrid strains for squalene production,” Journal of Applied Phycology, vol. 26, no. 1, pp. 29–41, 2014.

Aioi A., Shimizu T., Kuriyama K. Effect of squalene on superoxide anion generation induced by a skin irritant, lauroylsarcosine. Int. J. Pharm. 1995;113:159–164.

Bockisch, M. 1998. Fats and Oils Handbook, AOCS Press, Hamburg Gemany.

C. V. Rao, H. L. Newmark, and B. S. Reddy, “Chemopreventive effect of squalene on colon cancer,” Carcinogenesis, vol. 19, no. 2, pp. 287–290, 1998.

Clarke, S., E.J. Gulland, Milner, and T. Bjorndal. 2007. Perspectives: social, economic, and regulatory drivers of the shark fin trade, Marine Resource Economics, 22:305–327.

D. Mcphee, A. Pin, L. Kizer, and L. Perelman, “Squalane from sugarcane,” Cosmetics & Toiletries magazine, vol. 129, no. 6, pp. 1–6, 2014.

D. W. Hall, S. N. Marshall, K. C. Gordon, and D. P. Killeen, “Rapid quantitative determination of squalene in shark liver oils by Raman and IR spectroscopy,” Lipids, vol. 51, no. 1, pp. 139–147, 2016.

E. Naziri, F. Mantzouridou, and M. Z. Tsimidou, “Squalene resources and uses point to the potential of biotechnology,” Lipid Technology, vol. 23, no. 12, pp. 270–273, 2011.

F. Dent and S. Clarke, “State of the global market for shark products,” FAO Fishereis and Aquaculture Technical Paper, vol. 590, p. 187, 2015.

F. Esra Güneş, M. Üniversitesi Sağlık Bilimleri Fakültesi, B. Diyetetik Bölümü, and İ. Türkiye, “Medical use of squalene as a natural antioxidant derleme/review,” Journal of Marmara University Institute of Health Sciences, vol. 33, no. 44, pp. 220–228, 2013.

Fahmi, Dharmadi. Tinjauan Status Perikanan Hiu dan Upaya Konservasinya di Indonesia. Direktorat Konservasi Kawasan dan Jenis Ikan KKP, Juni 2013.

G. P. Ghimire, H. T. Nguyen, N. Koirala, and J. K. Sohng, “Advances in biochemistry and microbial production of squalene and its derivatives,” Journal of Microbiology and Biotechnology, vol. 26, no. 3, pp. 441–451, 2016.

G. S. Kelly, “Squalene and its potential clinical uses,” Alternative Medicine Review, vol. 44, no. 11, pp. 29–36, 1999.

G. Wejnerowska, P. Heinrich, and J. Gaca, “Separation of squalene and oil from Amaranthus seeds by supercritical carbon dioxide,” Separation and Purification Technology, vol. 110, pp. 39–43, 2013.

H. L. Newmark, “Squalene, olive oil, and and cancer risk: a review and hypoyhesis,” Carcinogenesis, vol. 6, no. 12, pp. 1101–1103, 1997.

H. P. He and H. Corke, “Oil and squalene in Amaranthus grain and leaf,” Journal of Agricultural and Food Chemistry, vol. 51, no. 27, pp. 7913–7920, 2003.

Indra T. Maulana1, Sukraso, dan Sophi Damayanti. Kandungan Asam Lemak Dalam Minyak Ikan Indonesia. Jurnal Ilmu dan Teknologi Kelautan Tropis, Vol. 6, No. 1, Hlm. 121-130, Juni 2014.

K. A. Mountfort, H. Bronstein, N. Archer, and S. M. Jickells, “Identification of oxidation products of squalene in solution and in latent fingerprints by ESI-MS and LC/APCI-MS,” Analytical Chemistry, vol. 79, no. 7, pp. 2650–2657, 2007.

K. H. S. Farvin, R. Anandan, S. Hari Senthil Kumar et al., “Cardioprotective effect of squalene on lipid profile in isoprenaline-induced myocardial infarction in rats,” Journal of Medicinal Food, vol. 9, no. 4, pp. 531–536, 2006.

Kohno Y., Egawa Y., Itoh S., Nagaoka S., Takahashi M., Mukai K. Kinetic study of quenching reaction of singlet oxygen and scavenging reaction of free radical by squalene in n-butanol. Biochim. Biophys. Acta. 1995;1256:52–56.

L. H. Reddy and P. Couvreur, “Squalene: a natural triterpene for use in disease management and therapy,” Advanced Drug Delivery Reviews, vol. 61, no. 15, pp. 1412–1426, 2009.

M. Azalia Lozano-Grande, Shela Gorinstein, Eduardo Espitia-Rangel, Gloria Dávila-Ortiz, and Alma Leticia Martínez-Ayala. Plant Sources, Extraction Methods, and Uses of Squalene. International Journal of Agronomy Volume 2018, 13 pages.

M. Tsujimoto, “Squalene: a highly unsaturated hydrocarbon in shark liver oil,” Industrial and Engineering Chemistry, vol. 12, no. 1, pp. 63–72, 1920.

O. Popa, N. E. Băbeanu, I. Popa, S. Niţă, and C. E. Dinu-Pârvu, “Methods for obtaining and determination of squalene from natural sources,” BioMed Research International, vol. 2015, Article ID 367202, 16 pages, 2015.

P. Gupta, K. Singhal, A. K. Jangra, V. Nautiyal, A. Pandey. Shark Liver Oil: A Review. AJPER 2012, Vol 1, Issue 2 (1-15)

Paolo M. Nainggolan. Pemburuan Hiu Ancaman Bagi Ekosistem Laut.https://www.national-oceanographic.com/article/pembu- ruan-hiu-ancaman-bagi-ekosistem-laut. 21 Jul 2021

R. Steiner, M. Drescher, M. Bonakdar, and W. Johannisbauer, Method for producing squalene, 2004.

R. W. Owen, R. Haubner, G. Würtele, W. E. Hull, B. Spiegelhalder, and H. Bartsch, “Olives and olive oil in cancer prevention,” European Journal of Cancer Prevention, vol. 13, no. 4, pp. 319–326, 2004.

Ryzard Amarowicz, “Squalene: a natural antioxidant?” European Journal of Lipid Science and Technology, vol. 111, no. 5, pp. 411-412, 2009.

S. K. Kim and F. Karadeniz, “Biological importance and applications of squalene and squalane,” Advances in Food and Nutrition Research, vol. 65, pp. 223–233, 2012.

T. J. Smith, “Squalene: potential chemopreventive agent,” Expert Opinion on Investigational Drugs, vol. 9, no. 8, pp. 1841–1848, 2000.

W. Xu, X. Ma, and Y. Wang, “Production of squalene by microbes: an update,” World Journal of Microbiology and Biotechnology, vol. 32, no. 12, 2016.

Z. R. Huang, Y. K. Lin, and J. Y. Fang, “Biological and pharmacological activities of squalene and related compounds: Potential uses in cosmetic dermatology,” Molecules, vol. 14, no. 1, pp. 540–554, 2009.